DNA-DNA hybridization assay for detection of Salmonella spp. in foods
نویسندگان
چکیده
منابع مشابه
Potential Use of DNA Aptamer-Magnetic Bead Separation-PCR Assay for Salmonella Detection in Food
Background: Salmonella is one of the most common food-borne pathogens that can cause illness. In this study, the sensitivity and the specificity of Aptamer-Magnetic bead Separation-Polymerase Chain Reaction (AMS-PCR) method were determined for Salmonella spp. detection. Methods: Different concentrations of Salmonella enterica were mixed with streptavidin-magnetic beads coated with biotinylated...
متن کاملDevelopment of a Sensitive Quantitative Competitive PCR Assay for Detection of Human Cytomegalovirus DNA
Accurate and rapid diagnosis of human cytomegalovirus (HCMV) disease in immunocompromised patients has remained as a challenge. Quantitative competitive PCR (QC-PCR) methods for detection of HCMV in these individuals have improved the positive and negative predictive values of PCR for diagnosis of HCMV disease. In this study we used QC-PCR assay, using a co-amplified DNA standard, to quantitate...
متن کاملDevelopment of a Differential PCR Assay for Detection of Brucella abortus and Brucella melitensis: an Analytical Approach for Monitoring of Brucella spp. in Foods of Animal Origin
Background: Classical bacteriological detection of Brucella species from food, and environment is routinely carried out based on morphological and biochemical characteristics. However, for increasing specificity and sensitivity of species identification methods, development of a molecular assay is necessary that was main aim of this study. Methods: Panel of some reference strains belonging to ...
متن کاملChemiluminescence dot blot hybridization assay for detection of B19 parvovirus DNA in human sera.
A chemiluminescence dot blot hybridization assay was used for the detection of B19 parvovirus DNA in human sera by using digoxigenin-labeled probes. The probes were revealed immunoenzymatically by use of anti-digoxigenin Fab fragments conjugated with alkaline phosphatase. The chemiluminescence signal was obtained by reacting the labeled probe-target complex with an enzyme-triggerable dioxetane ...
متن کاملAdvancement in electrochemical DNA-biosensors for GMOs detection: A review
Genetically modified organisms (GMOs) are plants or animals whose genetic make-up has been transformed by recombinant DNA technology, which has new features such as resistance to herbicides, virus and insect. Recently, genetic modification of food products has increased in order to reduce world poverty and hunger and increase food production However, the impact of GMOs on the human health is a ...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Applied and Environmental Microbiology
سال: 1983
ISSN: 0099-2240,1098-5336
DOI: 10.1128/aem.46.5.1146-1151.1983